Topic teaching: Genomics and Informatics broad strokes and how it ties into blood banking: Future at CBS

Presented by

Dr. Thomas Sierocinski
Bioinformatician
Canadian Blood Services, Vancouver

Dr. Celina Montemayor
Medical Officer
Canadian Blood Services, Toronto

Learning objectives

1. Describe high throughput methods for genomic testing
2. Discuss and compare the methods and their limitations in the context of Red Blood Cells typing
3. Estimate the technical requirements for high throughput data collection/experiments

References

1. Recent advances in blood group genotyping.   Lane WJ.  Ann Blood.  2021 Sept; 6:31  https://aob.amegroups.com/article/view/6473/html
2. Automated typing of red blood cell and platelet antigens: a whole-genome sequencing study.  Lane WJ, Westhoff CM, Gleadall NS, Aguad M, Smeland-Wagman R, Vege S, Simmons DP, Mah HH, Lebo MS, Walter K, Soranzo N, Di Angelantonio E, Danesh J, Roberts DJ, Watkins NA, Ouwehand WH, Butterworth AS, Kaufman RM, Rehm HL, Silberstein LE, Green RC; MedSeq Project.  Lancet Haematol. 2018 Jun;5(6):e241-e251. doi: 10.1016/S2352-3026(18)30053-X. Epub 2018 May 17.  PMID: 29780001
3. RBCeq: A robust and scalable algorithm for accurate genetic blood typing.  Jadhao S, Davison CL, Roulis EV, Schoeman EM, Divate M, Haring M, Williams C, Shankar AJ, Lee S, Pecheniuk NM, Irving DO, Hyland CA, Flower RL, Nagaraj SH.  EBioMedicine. 2022 Feb;76:103759. doi: 10.1016/j.ebiom.2021.103759. Epub 2022 Jan 14.  PMID: 35033986
4. BGvar: A comprehensive resource for blood group immunogenetics.  Rophina M, Pandhare K, Jadhao S, Nagaraj SH, Scaria V.  Transfus Med. 2022 Jun;32(3):229-236. doi: 10.1111/tme.12844. Epub 2021 Dec 13.  PMID: 34897852
5. Accurate long-read sequencing allows assembly of the duplicated RHD and RHCE genes harboring variants relevant to blood transfusion.  Zhang Z, An HH, Vege S, Hu T, Zhang S, Mosbruger T, Jayaraman P, Monos D, Westhoff CM, Chou ST.  Am J Hum Genet. 2022 Jan 6;109(1):180-191. doi: 10.1016/j.ajhg.2021.12.003. Epub 2021 Dec 29.  PMID: 34968422

Note: The session is a self-approved group learning activity (Section 1) as defined by the Maintenance of Certification program of The Royal College of Physicians and Surgeons of Canada.

Registration  

Closed

Appraisal

Closed

To be added to the distribution list for the LearnTransfusion series, send an email to learn.transfusion@blood.ca  with “Weekly Transfusion Medicine Seminars” in the subject line. Include your name and complete e-mail address in the body of the e-mail and confirm that you would like to be added to the distribution list for the LearnTransfusion series. You will then receive weekly email notifications of upcoming series events with details on how to join the webinars (e.g.,  link to register for the webinar).

The LearnTransfusion series is the seminar component of the Areas of Focused Competency (AFC)-Diploma Program in Transfusion Medicine. The series, organized by Canadian Blood Services, is also open to all practitioners in the field of Transfusion Medicine. Through a series of regular webinars, trainees and practitioners are exposed to various scientific, technical and clinical aspects of transfusion medicine.